Diagnostic Testing for Mpox

Available tests

• Mpox is diagnosed using real-time PCR tests. These tests are available through your local, state, territorial or tribal health department and many large commercial laboratories.
• Clinicians with specimens that are suspected to contain Monkeypox virus (MPXV) could contact their state or territorial public health department for information on testing options.
• Results are usually available in 2-4 days.
• Clinicians should collect two swabs from each lesion (generally from 2-3 lesions) in case additional testing, such as clade-specific testing, is needed for these patients.
• Clinicians should notify their state health department if they have a patient with mpox-like symptoms and recent travel to Central or Eastern Africa.

Steps

Specimen collection, storage, and shipping of human specimens are subject to Clinical Laboratory Improvement Amendments (CLIA) restrictions. Contact the laboratory testing facility to determine their specific requirements.

Refer to CDC's Specimen Collection Guidelines for general instructions on collecting, handling and shipping specimens.

1. Before testing, review CDC's Biosafety Laboratory Guidance for Handling and Processing Mpox Specimens for recommended laboratory procedures and biosafety guidelines when collecting, handling, and processing specimens.
2. Collect skin lesion material for initial laboratory testing. Once collected, these specimens can be tested at
   1. Commercial laboratories
   2. Laboratories within the Laboratory Response Network
3. While waiting for results, advise your patient to take precautions to avoid getting or spreading mpox to others.
4. To protect yourself and your staff, consult CDC's guidance on Infection Prevention and Control of Mpox in Healthcare Settings.