Notes from the Field: Escherichia coli  O157:H7 Outbreak in Children with Clostridioides difficile  Colonization Associated with an Improperly Treated Swimming Pool — Pennsylvania, June 2021

On June 7, 2021, the Pennsylvania Department of Health (PADOH) received multiple complaints of gastrointestinal illness from patrons of a community swimming pool. Two patrons reported positive Shiga toxin-producing Escherichia coli (STEC) and Clostridioides difficile from stool specimens. PADOH issued pool closure orders and initiated an outbreak response to identify a source and prevent additional illnesses.

Confirmed cases were defined as isolation of E. coli O157:H7 or detection of Shiga toxin or Shiga toxin genes from stool specimens of persons who visited the pool during May 31–June 7, 2021. Probable cases were defined as three or more loose stools in 24 hours with nausea, vomiting, fever, or cramps in persons who visited the pool during the same time frame. C. difficile results were deemed incidental upon consultation with experts (LC McDonald, MD, CDC, personal communication, June 2021) and were not included in the case definition.

Fifteen cases (nine confirmed, six probable) in persons aged 4–14 years were identified; 10 patients were male (Table). All persons reported swimming at the pool on May 31, 2021, the seasonal opening date, and had no other common exposures. The total number of pool visitors on this date is unknown. Symptom onsets occurred during June 2–June 4, 2021. Thirteen patients sought medical evaluation, and six were hospitalized. Four received antibiotics for C. difficile. None developed hemolytic uremic syndrome.

Early findings suggested an unusual association between exposure to a chlorinated swimming pool and infections caused by two pathogens susceptible to chlorine. Pool inspection revealed an automatic chlorinator malfunction. Record-keeping was inconsistent with local requirements, and the few available records demonstrated at least one instance of no detectable chlorine. The pool reopened following chlorinator repair, after which no additional cases were identified.

The investigation highlighted three important points regarding evaluation of outbreaks of childhood diarrheal disease. First, C. difficile testing is only recommended for children aged ≥2 years with prolonged or worsening diarrhea and risk factors, including immunocompromising conditions or relevant exposures (e.g., recent health care visits or antibiotics).* Reported prevalence of asymptomatic C. difficile colonization might vary by study population, laboratory detection method, and environmental setting. One study of children aged 1 month–12 years with diarrhea identified C. difficile toxin B in 3% of outpatients, 5% of inpatients, and 7% of asymptomatic controls (1). Recent studies using molecular techniques reported rates up to 25% in asymptomatic children aged 1–5 years (2) and 24% in persons aged 1–18 years without diarrhea (3). In the current outbreak, all children were previously healthy and considered to be at low risk for C. difficile infection. Thus, C. difficile testing was not indicated and provided no relevant clinical or epidemiologic data. Second, laboratory reports should include age-based interpretive suggestions for colonization versus infection and reminders that clinical symptoms are required for a diagnosis of C. difficile infection. Provider interpretations should include clinical and epidemiologic information. Finally, antibiotics are usually not required for treatment of diarrheal illnesses. In this STEC outbreak, no adverse outcomes were reported among the children receiving antibiotics. However, among STEC-infected persons, current guidance recommends against antibiotic use because of the risk for hemolytic uremic syndrome (4).

Enteric disease outbreaks caused by multiple pathogens rarely occur. Coinfections with C. difficile and other pathogens are unusual, but possible (5). Full investigation revealed that this outbreak was likely the result of STEC infections among children, some of whom were colonized with C. difficile. Recreational waters should be properly treated and maintained,^† and persons experiencing diarrhea should abstain from swimming.

Acknowledgments

Sameera Sayeed, Lisa Dettinger, Bureau of Laboratories, Pennsylvania Department of Health; staff members, Bureau of Community Health Systems, Pennsylvania Department of Health.

Corresponding author: Molly E. Nace, monace@pa.gov, 724-217-3337.

¹Bureau of Epidemiology, Pennsylvania Department of Health.

References

1. Cerquetti M, Luzzi I, Caprioli A, Sebastianelli A, Mastrantonio P. Role of Clostridium difficile in childhood diarrhea. Pediatr Infect Dis J 1995;14:598–603. https://doi.org/10.1097/00006454-199507000-00009 PMID:7567289
2. Merino VR, Nakano V, Finegold SM, Avila-Campos MJ. Genes encoding toxin of Clostridium difficile in children with and without diarrhea. Scientifica (Cairo) 2014;2014:594014. Epub April 29, 2014. https://doi.org/10.1155/2014/594014 PMID:24876992
3. Leibowitz J, Soma VL, Rosen L, Ginocchio CC, Rubin LG. Similar proportions of stool specimens from hospitalized children with and without diarrhea test positive for Clostridium difficile. Pediatr Infect Dis J 2015;34:261–6. https://doi.org/10.1097/INF.0000000000000556 PMID:25247582
4. Shane AL, Mody RK, Crump JA, et al. 2017 Infectious Diseases Society of America clinical practice guidelines for the diagnosis and management of infectious diarrhea. Clin Infect Dis 2017;65:e45–80. https://doi.org/10.1093/cid/cix669 PMID:29053792
5. Valentini D, Vittucci AC, Grandin A, et al. Coinfection in acute gastroenteritis predicts a more severe clinical course in children. Eur J Clin Microbiol Infect Dis 2013;32:909–15. https://doi.org/10.1007/s10096-013-1825-9 PMID:23370970

Abbreviations: EIA = enzyme immunoassay; GDH = glutamate dehydrogenase; Neg = negative; NT = not tested; PCR = polymerase chain reaction; Pos = positive.
* Type of testing performed varied by laboratory. The laboratories testing patients A, C, G, I, M, and N performed C. difficile GDH and toxin EIA, with reflex to PCR when GDH and toxin results were discordant. The laboratory testing Patient B, who had the first reported case, performed C. difficile toxin DNA PCR testing first. When the test resulted positive, toxin EIA was then performed.
^† Patient was presumed to be colonized with C. difficile.
^§ Patients D and E, I and J, K and L, and N and O are sibling pairs.
^¶ Patient received a diagnosis of appendicitis and received 1 dose of preoperative cefoxitin before the appendectomy.