Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 30, Number 4—April 2024
Dispatch

Isolation of Batborne Neglected Zoonotic Agent Issyk-Kul Virus, Italy

Davide LelliComments to Author , Ana Moreno, Sabrina Canziani, Laura Soliani, Maya Carrera, Anna Castelli, Francesca Faccin, Tiziana Trogu, Enrica Sozzi, Gian Luca Cavallari, Matteo Mauri, Fabiana Ferrari, Cristian Salogni, Chiara Garbarino, Chiara Chiapponi, Marco Farioli, and Antonio Lavazza
Author affiliations: University of Parma via Gramsci, Parma, Italy (D. Lelli); Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy (D. Lelli, A. Moreno, S. Canziani, L. Soliani, M. Carrera, A. Castelli, F. Faccin, T. Trogu, E. Sozzi, C. Salogni, C. Garbarino, C. Chiapponi, A. Lavazza); Wildlife Rehabilitation Center WWF of Valpredina, Cenate Sopra, Italy (G.L. Cavallari, M. Mauri); Piacenza Wildlife Rescue Centre, Piacenza, italy (F. Ferrari); DG Welfare Regione Lombardia, Milano, Italy (M. Farioli)

Main Article

Figure 2

Phylogenetic analysis of isolates from study of batborne neglected zoonotic agent Issyk-Kul virus, Italy A) Phylogeneny nairovirus protein sequences for Orthonairovirus large (L) segments, including the complete sequence obtained from Hypsugo savii bats, highlighted in yellow. Sequence colors were based on the genogroups proposed by Ozeki et al. 2022 (12). B) Nucleotide alignment magnification of a short PCR-targeted region of the L segment, encompassing all sequences derived from bat surveillance conducted during 2017–2023 identified as Issyk-Kul virus IT-297348-34/2022. Numbers along branches indicate bootstrap values.

Figure 2. Phylogenetic analysis of isolates from study of batborne neglected zoonotic agent Issyk-Kul virus, Italy A) Phylogeneny nairovirus protein sequences for Orthonairovirus large (L) segments, including the complete sequence obtained from Hypsugo savii bats, highlighted in yellow. Sequence colors were based on the genogroups proposed by Ozeki et al. 2022 (12). B) Nucleotide alignment magnification of a short PCR-targeted region of the L segment, encompassing all sequences derived from bat surveillance conducted during 2017–2023 identified as Issyk-Kul virus IT-297348-34/2022. Numbers along branches indicate bootstrap values.

Main Article

References
  1. Lvov  DK, Karas  FR, Timofeev  EM, Tsyrkin  YM, Vargina  SG, Veselovskaya  OV, et al. “Issyk-Kul” virus, a new arbovirus isolated from bats and Argas (Carios) vespertilionis (Latr., 1802) in the Kirghiz S.S.R. Brief report. Arch Gesamte Virusforsch. 1973;42:2079. DOIPubMedGoogle Scholar
  2. L’vov  DK, Kostiukov  MA, Daniiarov  OA, Tukhtaev  TM, Sherikov  BK. [Outbreak of arbovirus infection in the Tadzhik SSR due to the Issyk-Kul virus (Issyk-Kul fever)] [in Russian]. Vopr Virusol. 1984;29:8992.PubMedGoogle Scholar
  3. Atkinson  B, Marston  DA, Ellis  RJ, Fooks  AR, Hewson  R. Complete genomic sequence of Issyk-Kul virus. Genome Announc. 2015;3:e0066200715. DOIPubMedGoogle Scholar
  4. Brinkmann  A, Kohl  C, Radonić  A, Dabrowski  PW, Mühldorfer  K, Nitsche  A, et al. First detection of bat-borne Issyk-Kul virus in Europe. Sci Rep. 2020;10:22384. DOIPubMedGoogle Scholar
  5. Vargina  SG, Kuchuk  LA, Gershtein  VI, Karas  FR. Transmission of Issyk Kul virus by Argas vespertilionis ticks in experiment [in Russian]. Sborn nauch Tr Inst Virus Im Ivanov Akad Med Nauk SSSR. 1982;123–7.
  6. Bulychev  VP, Alekseev  AN, Kostiukov  MA, Gordeeva  ZE, L’vov  DK. [Issyk-Kul virus transmission by Aedes caspius caspius Pall. mosquitoes via experimental bite] [in Russian]. Med Parazitol (Mosk). 1979;48:536.PubMedGoogle Scholar
  7. Kostiukov  MA, Bulychev  VP, Lapina  TF. [Experimental infection of Aedes caspius caspius Pall. mosquitoes on dwarf bats, Vespertilio pipistrellus, infected with the Issyk-Kul virus and its subsequent transmission to susceptible animals] [in Russian]. Med Parazitol (Mosk). 1982;51:789.PubMedGoogle Scholar
  8. Cholleti  H, de Jong  J, Blomström  AL, Berg  M. Investigation of the virome and characterization of Issyk-Kul virus from Swedish Myotis brandtii bats. Pathogens. 2022;12:12. DOIPubMedGoogle Scholar
  9. Gigante  CM, Dettinger  L, Powell  JW, Seiders  M, Condori  REC, Griesser  R, et al. Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics. PLoS One. 2018;13:e0197074. DOIPubMedGoogle Scholar
  10. Lelli  D, Prosperi  A, Moreno  A, Chiapponi  C, Gibellini  AM, De Benedictis  P, et al. Isolation of a novel Rhabdovirus from an insectivorous bat (Pipistrellus kuhlii) in Italy. Virol J. 2018;15:37. DOIPubMedGoogle Scholar
  11. Walker  PJ, Widen  SG, Wood  TG, Guzman  H, Tesh  RB, Vasilakis  N. A global genomic characterization of nairoviruses identifies nine discrete genogroups with distinctive structural characteristics and host-vector associations. Am J Trop Med Hyg. 2016;94:110722. DOIPubMedGoogle Scholar
  12. Ozeki  T, Abe  H, Ushijima  Y, Nze-Nkogue  C, Akomo-Okoue  EF, Ella  GWE, et al. Identification of novel orthonairoviruses from rodents and shrews in Gabon, Central Africa. J Gen Virol. 2022;103:103. DOIPubMedGoogle Scholar
  13. Lv  X, Liu  Z, Li  L, Xu  W, Yuan  Y, Liang  X, et al. Yezo virus infection in tick-bitten patient and ticks, northeastern China. Emerg Infect Dis. 2023;29:797800. DOIPubMedGoogle Scholar
  14. Shayan  S, Bokaean  M, Shahrivar  MR, Chinikar  S. Crimean-Congo hemorrhagic fever. Lab Med. 2015;46:1809. DOIPubMedGoogle Scholar
  15. Sugimoto  S, Suda  Y, Nagata  N, Fukushi  S, Yoshikawa  T, Kurosu  T, et al. Characterization of Keterah orthonairovirus and evaluation of therapeutic candidates against Keterah orthonairovirus infectious disease. Ticks Tick Borne Dis. 2022;13:101834. DOIPubMedGoogle Scholar

Main Article

Page created: March 01, 2024
Page updated: March 20, 2024
Page reviewed: March 20, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external