The National Institute for Occupational Safety and Health (NIOSH)

Ethane, 1,1,1,2-tetrachloro-

RTECS #

KI8450000

CAS #

630-20-6
See: NMAM or OSHA Methods

Updated

March 2018

Molecular Weight

167.84

Molecular Formula

C₂H₂Cl₄

Synonyms

1,1,1,2-Tetrachloroethane
NCI-C52459
RCRA waste number U208

Skin and Eye Irritation and References

Route/Organism	Dose	Effect	Reference
eye /rabbit	100 mg	severe	AMPMAR 35,593,1974
skin /rabbit	500 mg/24H		AMPMAR 35,593,1974

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
gene conversion and mitotic recombination	/Aspergillus nidulans	250 ppm	MUREAV 201,401,1988
mutation in mammalian somatic cells	lymphocyte/mouse	200 mg/L	EMMUEG 12,85,1988
mutation in microorganisms	lymphocyte/mouse	200 mg/L (+enzymatic activation step)	SCIEAS 236,933,1987
mutation in microorganisms	/Salmonella typhimurium	10 µg/plate (+/-enzymatic activation step)	TECSDY 15,101,1987
other mutation test systems	oral/mouse	1 gm/kg	MUREAV 343,157,1995
sex chromosome loss and nondisjunction	/Aspergillus nidulans	125 ppm	MUREAV 201,401,1988
sex chromosome loss and nondisjunction	lung/human	100 mg/L	MUREAV 369,243,1996
sister chromatid exchange	ovary/hamster	158 mg/L	SCIEAS 236,933,1987
specific locus test	ovary/hamster	0.65 gm/L/24H	MUREAV 602,14,2006

Tumorigenic Data and References

Route/Organism	Dose	Effect	Reference
oral/mouse	lowest published toxic dose: 128750 mg/kg/103W- intermittent	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors	NTIS** PB83-218206
oral/rat	lowest published toxic dose: 128750 mg/kg/103W- intermittent	Tumorigenic: Equivocal tumorigenic agent by RTECS criteria Liver: Tumors	NTIS** PB83-218206
oral/rat	lowest published toxic dose: 64375 mg/kg/103W- intermittent	Tumorigenic: Neoplastic by RTECS criteria Skin and Appendages: Tumors	NTIS** PB83-218206

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
In Vitro/Mouse, liver	Inhibitor Concentration (50 percent kill): 0.7 mmol/L/2H	In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)	TIVIEQ 3,145,1989
In Vitro/Rat, liver	Inhibitor Concentration (50 percent kill): 1.4 mmol/L/2H	In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)	TIVIEQ 3,145,1989
inhalation/rabbit	lowest published toxic concentration: 2000 ppm	Lung, Thorax, or Respiration: Acute pulmonary edema Lung, Thorax, or Respiration: Cough	VCVGH* -,384,1990
inhalation/rabbit	lowest published toxic concentration: 2500 ppm	Lung, Thorax, or Respiration: Acute pulmonary edema Liver: Hepatitis (hepatocellular necrosis), zonal Blood: Hemorrhage	VCVGH* -,384,1990
inhalation/rabbit	lethal concentration (50 percent kill): 2800 ppm/4H	Gastrointestinal: Hypermotility, diarrhea	AMPMAR 35,593,1974
inhalation/rabbit	lethal concentration (50 percent kill): 2800 mg/m³/4H		VCVGH* -,383,1990
inhalation/rat	lethal concentration (50 percent kill): 2100 mg/m³/4H		VCVGH* -,383,1990
inhalation/rat	lethal concentration (50 percent kill): 2100 ppm/4H	Gastrointestinal: Hypermotility, diarrhea	AMPMAR 35,593,1974
inhalation/rat	lowest published toxic concentration: 2000 ppm	Lung, Thorax, or Respiration: Cough	VCVGH* -,384,1990
inhalation/rat	lowest published toxic concentration: 3000 ppm	Lung, Thorax, or Respiration: Acute pulmonary edema	VCVGH* -,384,1990
inhalation/rat	lowest published toxic concentration: 4000 ppm	Lung, Thorax, or Respiration: Acute pulmonary edema Liver: Other changes	VCVGH* -,384,1990
intraperitoneal/mouse	lethal dose (50 percent kill): 1275 mg/kg		SAIGBL 8,371,1966
oral/guinea pig	lowest published toxic dose: 600 mg/kg	Liver: Hepatitis (hepatocellular necrosis), zonal	VCVGH* -,384,1990
oral/mouse	lowest published toxic dose: 1000 mg/kg	Liver: Hepatitis (hepatocellular necrosis), zonal	VCVGH* -,384,1990
oral/mouse	lethal dose (50 percent kill): 1500 mg/kg		VCVGH* -,383,1990
oral/rat	lethal dose (50 percent kill): 670 mg/kg		VCVGH* -,383,1990
skin/rabbit	lethal dose (50 percent kill): 20 mg/kg		VCVGH* -,383,1990

Other Multiple Dose Data and References

Route/Organism	Dose	Effect	Reference
intraperitoneal/mouse	lowest published toxic dose: 16 gm/kg/40D- intermittent	Behavioral: Sleep	SAIGBL 8,371,1966
intraperitoneal/mouse	lowest published toxic dose: 2400 mg/kg/3D- intermittent	Behavioral: Sleep Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	SAIGBL 8,371,1966
oral/mouse	lowest published toxic dose: 14 gm/kg/14D- continuous	Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	NTPTR* NTP-TR-237,1982
oral/mouse	lowest published toxic dose: 32500 mg/kg/13W- intermittent	Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	NTPTR* NTP-TR-237,1982
oral/rat	lowest published toxic dose: 128750 mg/kg/103W- intermittent	Brain and Coverings: Tumors Tumorigenic: Active as anti-cancer agent	NTIS** PB83-218206
oral/rat	lowest published toxic dose: 7 gm/kg/14D- continuous	Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	NTPTR* NTP-TR-237,1982

Reviews

Organization	Standard	Reference
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Limited Evidence	IMEMDT 41,87,1986
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Limited Evidence	IMEMDT 71,1133,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Sufficient Evidence	IMEMDT 106,487,2014
International Agency for Research on Cancer (IARC)	Cancer Review:Human No Adequate Data	IMEMDT 41,87,1986
International Agency for Research on Cancer (IARC)	Cancer Review:Human No Adequate Data	IMEMDT 71,1133,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Human Inadequate Evidence	IMEMDT 106,487,2014
International Agency for Research on Cancer (IARC)	Cancer Review:Group 3	IMEMDT 71,1133,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Group 2B	IMEMDT 106,487,2014
TOXICOLOGY REVIEW		AIHAAP 40(3),A46,1979
TOXICOLOGY REVIEW		MUREAV 543,201,2003
TOXICOLOGY REVIEW		MUTAEX 14,5,1999
TOXICOLOGY REVIEW		MUREAV 628,67,2007
TOXICOLOGY REVIEW		MUREAV 584,1,2005
TOXICOLOGY REVIEW		MUREAV 769,34,2014
TOXICOLOGY REVIEW		MUREAV 802,1,2016
TOXICOLOGY REVIEW		MUTAEX 31,347,2016

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO 1,1,1,2-TETRACHLOROETHANE-air	Handle with caution	NIOSH* DHHS #92-100,1992

Status in Federal Agencies

Organization	Reference
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Studies (gavage);clear evidence:mouse
NCI Carcinogenesis Studies (gavage);equivocal evidence:rat
NIOSH CURRENT INTELLIGENCE BULLETIN 27, 1978
NTP Toxicity studies, RPT# TOX-45, October 2000
On EPA IRIS database

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health

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