1,3-Butadiene, hexachloro-
RTECS #
EJ0700000
CAS #
Updated
October 2017
Molecular Weight
260.74
Molecular Formula
C4Cl6
Synonyms
1,1,2,3,4,4-Hexachloro-1,3-butadiene
1,3-Hexachlorobutadiene
Dolen-pur
GP-40-66:120
HCBD
Hexachlor-1,3-butadien (Czech)
Hexachlorbutadiene
Hexachloro-1,3-butadiene
Hexachlorobutadiene
Hexachlorobutadiene (ACGIH)
Perchlorobutadiene
RCRA waste number U128
1,3-Hexachlorobutadiene
Dolen-pur
GP-40-66:120
HCBD
Hexachlor-1,3-butadien (Czech)
Hexachlorbutadiene
Hexachloro-1,3-butadiene
Hexachlorobutadiene
Hexachlorobutadiene (ACGIH)
Perchlorobutadiene
RCRA waste number U128
Skin and Eye Irritation and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| eye /rabbit | 162 mg | mild | EJTXAZ 9,171,1976 |
| eye /rabbit | 500 mg/24H | mild | 85JCAE -,116,1986 |
| skin /rabbit | 810 mg/24H | moderate | EJTXAZ 9,171,1976 |
| skin /rabbit | 500 mg/24H | mild | 85JCAE -,116,1986 |
Mutation Data and Reference
| System Test | Route/Organism/Tissue | Dose | Reference |
|---|---|---|---|
| Cytogenetic Analysis | lymphocyte/human | 1 mg/L | CYGEDX 22(4),46,1988 |
| Cytogenetic Analysis | oral/mouse | 2 mg/kg | CYGEDX 22(4),46,1988 |
| Cytogenetic Analysis | inhalation/mouse | 10 mg/m3 | CYGEDX 22(4),46,1988 |
| Cytogenetic Analysis | lung/hamster | 6.3 mg/L/4H (+/-enzymatic activation step) | MUREAV 699,47,2010 |
| DNA Damage | liver/human | 10 µmol/l/48H | MUREAV 696,21,2010 |
| DNA Damage | liver/human | 1 mmol/l/48H | MUREAV 696,21,2010 |
| DNA Damage | liver/human | 1 µmol/l/48H | MUREAV 696,21,2010 |
| morphological transform | embryo/hamster | 10 mg/L | CALEDQ 23,297,1984 |
| mutation in microorganisms | /Salmonella typhimurium | 1 mg/plate (+enzymatic activation step) | DHEFDK FDA-78-1046,1978 |
| mutation in microorganisms | /Salmonella typhimurium | 320 µg/plate (-enzymatic activation step) | CRNGDP 7,431,1986 |
| sister chromatid exchange | ovary/hamster | 1400 µg/L | EMMUEG 10(Suppl 10),1,1987 |
| Unscheduled DNA Synthesis | embryo/hamster | 2 mg/L | CALEDQ 23,297,1984 |
| Unscheduled DNA Synthesis | oral/rat | 77 gm/kg/11W | TXAPA9 60,287,1981 |
Reproductive Effects Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| inhalation/rat | 15 ppm/6H (6-20D pregnant) | Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) | TOLED5 47,235,1989 |
| intraperitoneal/rat | 150 mg/kg (1-15D pregnant) | Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) | SWEHDO 7(Suppl 4),66,1981 |
| oral/rat | 45 mg/kg (13W male/13W pre-3W after birth) | Reproductive: Effects on newborn: Biochemical and metabolic | TXAPA9 42,387,1977 |
| oral/rat | 4 gm/kg (13W male/13W pre-3W after birth) | Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) | TXAPA9 42,387,1977 |
| oral/rat | 178 mg/kg (1-22D pregnant) | Reproductive: Specific developmental abnormalities: Central nervous system Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) | AAGEAA 89(8),44,1985 |
| oral/mouse | 10920 µg/kg (13W male) | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) | NTIS** PB91-185884 |
| oral/rat | 20 mg/kg (multigenerations) | Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) | NTIS** #PB93-123867 |
| oral/rat | 75 mg/kg (multigenerations) | Reproductive: Specific developmental abnormalities: Urogenital system Reproductive: Effects on newborn: Biochemical and metabolic | NTIS** #PB93-123867 |
| oral/rat | 225 mg/kg (17-22D pregnant) | Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth) | NTIS** #PB93-123867 |
| oral/rat | 37.5 mg/kg (multigenerations) | Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive) Reproductive: Effects on newborn: Biochemical and metabolic | NTIS** #PB93-123867 |
| oral/rat | 5 mg/kg (multigenerations) | Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) | NTIS** #PB93-123867 |
| subcutaneous/rat | 20 mg/kg (1D prior to copulation) | Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4) | GISAAA 31,33,1966 |
Tumorigenic Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| oral/rat | lowest published toxic dose: 15 gm/kg/2Y- continuous | Tumorigenic: Carcinogenic by RTECS criteria Kidney, Ureter, and Bladder: Kidney tumors | AIHAAP 38,589,1977 |
Acute Toxicity Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| In Vitro/Human, liver tumor | Inhibitor Concentration Low: 10 µmol/L | In Vitro Toxicity Studies: Other assays | TIVIEQ 26,511,2012 |
| In Vitro/Rat, liver | Inhibitor Concentration Low: 31.6 µmol/L | In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc. | TIVIEQ 26,511,2012 |
| inhalation/mammal (species unspecified) | lethal concentration (50 percent kill): 370 mg/m3 | GISAAA 55(5),72,1990 | |
| inhalation/mouse | lethal concentration (50 percent kill): 370 mg/m3 | GISAAA 37(2),32,1972 | |
| inhalation/mouse | lethal concentration (50 percent kill): 370 mg/m3/2H | VCVGH* -,518,1990 | |
| inhalation/rat | lethal concentration (50 percent kill): 630 mg/m3/4H | VCVGH* -,518,1990 | |
| intraperitoneal/mouse | lethal dose (50 percent kill): 76 mg/kg | EJTXAZ 7,247,1974 | |
| intraperitoneal/mouse | lowest published toxic dose: 25 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) | VCVGH* -,519,1990 |
| intraperitoneal/rat | lowest published toxic dose: 300 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Other changes in urine composition | VCVGH* -,519,1990 |
| intraperitoneal/rat | lethal dose (50 percent kill): 175 mg/kg | Behavioral: Somnolence (general depressed activity) Behavioral: Irritability | EJTXAZ 8,180,1975 |
| intraperitoneal/rat | lowest published toxic dose: 50 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) | TOLED5 182,84,2008 |
| intraperitoneal/rat | lowest published toxic dose: 100 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Biochemical: Metabolism (intermediary): Other proteins | TOLED5 182,84,2008 |
| intraperitoneal/rat | lowest published toxic dose: 100 mg/kg | Kidney, Ureter, and Bladder: Other changes | TOLED5 172S,S1,2007 |
| intraperitoneal/rat | lowest published toxic dose: 100 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Renal function tests depressed | TOLED5 172S,S1,2007 |
| oral/cat | lethal dose (50 percent kill): 187 mg/kg | VCVGH* -,519,1990 | |
| oral/guinea pig | lethal dose (50 percent kill): 90 mg/kg | ENTOX* -,513,2005 | |
| oral/guinea pig | lowest published toxic dose: 10 mg/kg | Nutritional and Gross Metabolic: Conditioned vitamin deficiency | VCVGH* -,519,1990 |
| oral/hamster | lethal dose (50 percent kill): 960 mg/kg | TXAPA9 48,A192,1979 | |
| oral/mouse | lethal dose (50 percent kill): 51 mg/kg | VCVGH* -,518,1990 | |
| oral/rat | lethal dose (50 percent kill): 82 mg/kg | GISAAA 37(2),32,1972 | |
| oral/rat | lowest published toxic dose: 8.1 mg/kg | Spinal Cord: Other degenerative changes | VCVGH* -,520,1990 |
| oral/rat | lowest published toxic dose: 10 mg/kg | Nutritional and Gross Metabolic: Conditioned vitamin deficiency | VCVGH* -,519,1990 |
| oral/rat | lowest published toxic dose: 200 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Other changes in urine composition Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Peptidases | TXAPA9 132,203,1995 |
| skin/mouse | lethal dose (50 percent kill): 150 mg/kg | VCVGH* -,518,1990 | |
| skin/rabbit | lethal dose (50 percent kill): 100 mg/kg | GISAAA 37(2),32,1972 | |
| skin/rabbit | lethal dose (50 percent kill): 126 mg/kg/7H | ENTOX* -,513,2005 | |
| skin/rat | lethal dose (50 percent kill): 4500 mg/kg | VCVGH* -,519,1990 | |
| unreported route/mammal (species unspecified) | lethal dose (50 percent kill): 200 mg/kg | 30ZDA9 -,40,1971 | |
| unreported route/rat | lowest published toxic dose: 200 mg/kg | Kidney, Ureter, and Bladder: Other changes in urine composition | TOXID9 44,104,2005 |
Other Multiple Dose Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| inhalation/man | lowest published toxic concentration: 0.02 ppm/9Y- intermittent | Liver: Change in gall bladder structure or function Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) | BJIMAG 49,700,1992 |
| inhalation/rat | lowest published toxic concentration: 26 mg/m3/2H/30D- intermittent | Blood: Other changes Blood: Changes in leukocyte (WBC) count Nutritional and Gross Metabolic: Weight loss or decreased weight gain | GISAAA 37(2),32,1972 |
| inhalation/rat | lowest published toxic concentration: 100 ppm/6H/2W- intermittent | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Nutritional and Gross Metabolic: Weight loss or decreased weight gain Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | BJIMAG 27,1,1970 |
| intraperitoneal/rat | lowest published toxic dose: 50 mg/kg/2D- intermittent | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Renal function tests depressed Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases | CBCPAI 136,367,2003 |
| intraperitoneal/rat | lowest published toxic dose: 75 mg/kg/3D- intermittent | Behavioral: Tolerance Kidney, Ureter, and Bladder: Other changes Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | CBCPAI 136,367,2003 |
| intraperitoneal/rat | lowest published toxic dose: 100 mg/kg/4D- intermittent | Behavioral: Ataxia Behavioral: Tolerance Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | CBCPAI 136,367,2003 |
| intraperitoneal/rat | lowest published toxic dose: 313 mg/kg/3D- intermittent | Liver: Other changes Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases | TIHEEC 8,191,1992 |
| oral/guinea pig | lowest published toxic dose: 420 mg/kg/30W- intermittent | Liver: Other changes Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Immunological Including Allergic: Increase in cellular immune response | GTPZAB 9(11),50,1965 |
| oral/mouse | lowest published toxic dose: 540 mg/kg/15D- continuous | Cardiac: Changes in heart weight Endocrine: Changes in spleen weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain | JEPOEC 9,323,1989 |
| oral/mouse | lowest published toxic dose: 1092 mg/kg/13W- continuous | Cardiac: Changes in heart weight Kidney, Ureter, and Bladder: Changes in bladder weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain | NTIS** PB91-185884 |
| oral/rat | lowest published toxic dose: 1274 mg/kg/26W- intermittent | Brain and Coverings: Other degenerative changes Behavioral: Alteration of operant conditioning Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) | GTPZAB 11(3),23,1967 |
| oral/rat | lowest published toxic dose: 14600 mg/kg/2Y- continuous | Kidney, Ureter, and Bladder: Other changes in urine composition Kidney, Ureter, and Bladder: Other changes Nutritional and Gross Metabolic: Weight loss or decreased weight gain | EVHPAZ 21,49,1977 |
| oral/rat | lowest published toxic dose: 105 mg/kg/14D- continuous | Kidney, Ureter, and Bladder: Changes in bladder weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain | TXAPA9 47,1,1979 |
| oral/rat | lowest published toxic dose: 794 mg/kg/18W- intermittent | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Changes in bladder weight Liver: Changes in liver weight | TXAPA9 47,1,1979 |
| oral/rat | lowest published toxic dose: 80 mg/kg/16D- continuous | Behavioral: Food intake (animal) Kidney, Ureter, and Bladder: Other changes Nutritional and Gross Metabolic: Weight loss or decreased weight gain | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 160 mg/kg/16D- continuous | Behavioral: Tremor Behavioral: Muscle weakness | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 160 mg/kg/16D- continuous | Behavioral: Sleep | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 160 mg/kg/16D- continuous | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Changes in kidney weight | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 1040 mg/kg/16D- continuous | Kidney, Ureter, and Bladder: Other changes in urine composition | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 320 mg/kg/16D- continuous | Behavioral: Fluid intake | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 600 mg/kg/16D- continuous | Kidney, Ureter, and Bladder: Urine volume increased | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 385 mg/kg/7D- continuous | Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NTIS** #PB93-123867 |
| oral/rat | lowest published toxic dose: 560 mg/kg/4W- continuous | Kidney, Ureter, and Bladder: Other changes in urine composition Endocrine: Changes in adrenal weight Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases | FCTOD7 31,125,1993 |
Reviews
| Organization | Standard | Reference |
|---|---|---|
| American Conference of Governmental Industrial Hygienists (ACGIH) | Threshold Limit Value-time-weighted average 0.02 ppm (skin) | DTLVS* TLV/BEI,2013 |
| American Conference of Governmental Industrial Hygienists (ACGIH) | Threshold Limit Value-Confirmed animal carcinogen | DTLVS* TLV/BEI,2013 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Animal Limited Evidence | IMEMDT 20,179,1979 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Animal Limited Evidence | IMEMDT 73,277,1999 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Human No Adequate Data | IMEMDT 20,179,1979 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Human Inadequate Evidence | IMEMDT 73,277,1999 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Group 3 | IMEMDT 73,277,1999 |
| TOXICOLOGY REVIEW | ENTOX* -,513,2005 | |
| TOXICOLOGY REVIEW | MUREAV 584,1,2005 | |
| TOXICOLOGY REVIEW | BCLPT* 96,131,2005 | |
| TOXICOLOGY REVIEW | MUREAV 590,1,2005 | |
| TOXICOLOGY REVIEW | MUREAV 802,1,2016 |
Standards and Regulations
NIOSH Documentation and Surveillance
| Organization | Standard | Reference |
|---|---|---|
| National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO HEXACHLOROBUTADIENE-air | 10H Carcinogen time-weighted average 0.02 ppm (Sk) | NIOSH* DHHS #92-100,1992 |
| National Occupational Exposure Survey 1983 | Hazard Code X6278; Number of Industries 1; Total Number of Facilities 3; Number of Occupations 11; Total Number of Employees Exposed 1010; Total Number of Female Employees Exposed 26 |
Status in Federal Agencies
| Organization | Reference |
|---|---|
| ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/100160/AS) | |
| EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE | FEREAC 47,26992,82 |
| EPA TSCA Section 8(b) CHEMICAL INVENTORY | |
| EPA TSCA Section 8(d) unpublished health/safety studies | |
| EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001 | |
| NIOSH Analytical Method, 1994: Hexachlorobutadiene, 2543 | |
| NTP Toxicity studies, RPT# TOX-01, October 2000 | |
| On EPA IRIS database |
Page last reviewed: November 16, 2018
Content
source: National Institute for Occupational Safety and Health