Benzene, m-dinitro-
RTECS #
CZ7350000
CAS #
Updated
March 2019
Molecular Weight
168.12
Molecular Formula
C6H4N2O4
Synonyms
1,3-Dinitrobenzene
1,3-Dinitrobenzol
2,4-Dinitrobenzene
Benzene, 1,3-dinitro-
Binitrobenzene
Dwunitrobenzen (Polish)
m-Dinitrobenzene
m-Dinitrobenzene (ACGIH:OSHA)
1,3-Dinitrobenzol
2,4-Dinitrobenzene
Benzene, 1,3-dinitro-
Binitrobenzene
Dwunitrobenzen (Polish)
m-Dinitrobenzene
m-Dinitrobenzene (ACGIH:OSHA)
Skin and Eye Irritation and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| eye /rabbit | 100 mg | ATDAEI 1,168,1992 |
Mutation Data and Reference
| System Test | Route/Organism/Tissue | Dose | Reference |
|---|---|---|---|
| DNA Damage | testis/rat | 0.04 µmol/L | EMMUEG 44,179,2004 |
| mutation in microorganisms | /Salmonella typhimurium | 3300 ng/plate (+enzymatic activation step) | ENMUDM 2,531,1980 |
| mutation in microorganisms | /Salmonella typhimurium | 50 µg/plate (-enzymatic activation step) | MUREAV 44,9,1977 |
| other mutation test systems | /Salmonella typhimurium | 12500 µg/L | MUREAV 272,91,1992 |
Reproductive Effects Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| intraperitoneal/rat | 10 µg/kg (1D male) | Reproductive: Other effects on male | REPTED 14,45,2000 |
| oral/mouse | 48 mg/kg (1D male) | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct | JTEHD6 28,67,1989 |
| oral/rat | 24 mg/kg (1D male) | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct | FAATDF 14,284,1990 |
| oral/rat | 33600 µg/kg (16W prior to copulation) | Reproductive: Maternal effects: Ovaries, fallopian tubes | JTEHD6 7,829,1981 |
| oral/rat | 48 mg/kg (1D male) | Reproductive: Effects on fertility: Male fertility index (e.g., # males impregnating females per # males exposed to fertile nonpregnant females) | FAATDF 14,284,1990 |
| oral/rat | 90 mg/kg (12W male) | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) | JTEHD6 19,477,1986 |
Acute Toxicity Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| In Vitro/Human, endocrine tumor | Inhibitor Concentration Low: 50 µmol/L/48H | In Vitro Toxicity Studies: Other assays Endocrine: Estrogenic | TOLED5 213,275,2012 |
| In Vitro/paa | Inhibitor Concentration Low: 1 mmol/L/24H | NETEEC 32,362,2011 | |
| In Vitro/paa | Inhibitor Concentration Low: 1 mmol/L/45M | In Vitro Toxicity Studies: Other assays | NETEEC 32,362,2011 |
| In Vitro/paa | Inhibitor Concentration Low: 1 mmol/L/5H | NETEEC 32,362,2011 | |
| In Vitro/pax | Inhibitor Concentration Low: 1 mmol/L/48H | In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. | NRTXDN 37,74,2013 |
| In Vitro/pax | Inhibitor Concentration Low: 1 µmol/L/48H | In Vitro Toxicity Studies: Cell membrane integrity (prelabeled cells): release of radioactive isotopes ([51Cr], [3H]-thymidine, [3H]-proline, [35S]- or [75Se]-methionine, 5-[125I]-2-deoxy-uridine) or fluorescent dyes (bis-carboxyethyl-carboxyfluorescein (BCECF) or calcein-AM) | NRTXDN 37,74,2013 |
| In Vitro/Rat, testis | Inhibitor Concentration Low: 5 µmol/L/48H | Reproductive: Paternal effects: Testes, epididymis, sperm duct | TIVIEQ 1,31,1987 |
| intraperitoneal/mouse | lowest published lethal dose: 200 mg/kg | JAPMA8 48,419,1959 | |
| intraperitoneal/rat | lethal dose (50 percent kill): 28 mg/kg | AEPPAE 207,446,1949 | |
| intravenous/Dog | lethal dose (50 percent kill): 10 mg/kg | Peripheral Nerve and Sensation: Spastic paralysis with/without sensory change Behavioral: Convulsions or effect on seizure threshold Blood: Methemoglobinemia- Carboxhemoglobinemia | NTIS** AD-A066-307 |
| oral/cat | lowest published lethal dose: 27 mg/kg | Behavioral: Coma Lung, Thorax, or Respiration: Cyanosis Blood: Other hemolysis with or without anemia | LANCAO 2,582,1901 |
| oral/Dog | lowest published lethal dose: 600 mg/kg | NTIS** AD-A066-307 | |
| oral/human | lowest published lethal dose: 28 mg/kg | 34ZIAG -,226,1969 | |
| oral/mouse | lethal dose (50 percent kill): 74700 µg/kg | Behavioral: Somnolence (general depressed activity) Behavioral: Tremor Lung, Thorax, or Respiration: Respiratory stimulation | ATDAEI 1,168,1992 |
| oral/rabbit | lowest published lethal dose: 400 mg/kg | NTIS** AD-A066-307 | |
| oral/rat | lethal dose (50 percent kill): 59500 µg/kg | Behavioral: Somnolence (general depressed activity) Lung, Thorax, or Respiration: Dyspnea Skin and Appendages: Other: Hair | ATDAEI 1,168,1992 |
| oral/rat | lowest published toxic dose: 15 mg/kg | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct | TXAPA9 92,54,1988 |
| oral/rat | lowest published toxic dose: 50 mg/kg | Blood: Changes in spleen Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Related to Chronic Data: Changes in testicular weight | TXAPA9 92,54,1988 |
| oral/rat | lowest published toxic dose: 30 mg/kg | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Related to Chronic Data: Changes in testicular weight | ARTODN 66,435,1992 |
| oral/rat | lowest published toxic dose: 60 mg/kg | Kidney, Ureter, and Bladder: Other changes in urine composition Reproductive: Paternal effects: Testes, epididymis, sperm duct | ARTODN 66,435,1992 |
| oral/rat | lowest published toxic dose: 32 mg/kg | Endocrine: Androgenic Reproductive: Paternal effects: Testes, epididymis, sperm duct Related to Chronic Data: Changes in testicular weight | TXAPA9 95,255,1988 |
| oral/rat | lowest published toxic dose: 4 mg/kg | Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Biochemical: Metabolism (intermediary): Other | TOLED5 213,275,2012 |
| oral/rat | lowest published toxic dose: 25 mg/kg | Reproductive: Paternal effects: Testes, epididymis, sperm duct | TXCYAC 290,169,2011 |
| oral/wild bird | lethal dose (50 percent kill): 42 mg/kg | TXAPA9 21,315,1972 | |
| skin/man | lowest published toxic dose: 4 mg/kg/2D- intermittent | Behavioral: Change in motor activity (specific assay) Lung, Thorax, or Respiration: Cyanosis | LANCAO 2,582,1901 |
| skin/rabbit | lethal dose (50 percent kill): 1900 mg/kg | ATDAEI 1,168,1992 |
Other Multiple Dose Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| implant/rat | lowest published toxic dose: 30 mg/kg/6D- continuous | Brain and Coverings: Other degenerative changes Blood: Hemorrhage | TXAPA9 161,267,1999 |
| intraperitoneal/rat | lowest published toxic dose: 30 mg/kg/2D- intermittent | Brain and Coverings: Other degenerative changes Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductases Biochemical: Metabolism (intermediary): Other | TOXID9 44,380,2005 |
| intraperitoneal/rat | lowest published toxic dose: 30 mg/kg/4D- intermittent | Brain and Coverings: Other degenerative changes Behavioral: Ataxia Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NRTXDN 20,83,1999 |
| oral/rat | lowest published toxic dose: 16 mg/kg/4D- intermittent | Reproductive: Paternal effects: Testes, epididymis, sperm duct Biochemical: Metabolism (intermediary): Other | TOLED5 213,275,2012 |
| oral/rat | lowest published toxic dose: 32 mg/kg/4D- intermittent | Related to Chronic Data: Changes in testicular weight | TOLED5 213,275,2012 |
| oral/rat | lowest published toxic dose: 700 mg/kg/2W- intermittent | Reproductive: Paternal effects: Testes, epididymis, sperm duct Related to Chronic Data: Changes in testicular weight | JTSCDR 25,251,2000 |
| oral/rat | lowest published toxic dose: 156 mg/kg/90D- intermittent | Endocrine: Changes in spleen weight Blood: Methemoglobinemia- Carboxhemoglobinemia | TOXID9 30,200,1996 |
| oral/rat | lowest published toxic dose: 265 mg/kg/8W- continuous | Blood: Pigmented or nucleated red blood cells Nutritional and Gross Metabolic: Weight loss or decreased weight gain Related to Chronic Data: Changes in testicular weight | JTEHD6 7,829,1981 |
| oral/rat | lowest published toxic dose: 296 mg/kg/16W- continuous | Endocrine: Changes in spleen weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain Related to Chronic Data: Changes in testicular weight | JTEHD6 7,829,1981 |
| unreported route/rat | lowest published toxic dose: 9 mg/kg/3D- intermittent | Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct Biochemical: Metabolism (intermediary): Other | TOXID9 90,219,2006 |
Reviews
| Organization | Standard | Reference |
|---|---|---|
| American Conference of Governmental Industrial Hygienists (ACGIH) | Threshold Limit Value-time-weighted average 0.15 ppm (skin) | DTLVS* TLV/BEI,2013 |
| TOXICOLOGY REVIEW | JTSCDR 25,1,2000 | |
| TOXICOLOGY REVIEW | TOLED5 140-141,105,2003 | |
| TOXICOLOGY REVIEW | MUREAV 567,347,2004 | |
| TOXICOLOGY REVIEW | REPTED 26,185,2008 | |
| TOXICOLOGY REVIEW | RTOPDW 60,120,2011 |
Standards and Regulations
| Organization | Standard | Reference |
|---|---|---|
| Mine Safety and Health Administration (MSHA) STANDARD-air | time-weighted average 0.15 ppm (1 mg/m3) (skin) | DTLVS* 3,92,1971 |
| Occupational Exposure Limit-AUSTRALIA | time-weighted average 0.15 ppm (1 mg/m3), JUL2008 | |
| Occupational Exposure Limit-DENMARK | time-weighted average 0.15 ppm (1 mg/m3), skin, MAY2011 | |
| Occupational Exposure Limit-FINLAND | time-weighted average 1 mg/m3, short term exposure limit 3 mg/m3, skin, NOV2011 | |
| Occupational Exposure Limit-FRANCE | VME 0.15 ppm (1 mg/m3), FEB2006 | |
| Occupational Exposure Limit-HUNGARY | time-weighted average 1 mg/m3, short term exposure limit 2 mg/m3, Skin, SEP2000 | |
| Occupational Exposure Limit-ICELAND | time-weighted average 0.15 ppm (1 mg/m3), skin, NOV2011 | |
| Occupational Exposure Limit-JAPAN | Occupational Exposure Limit 0.15 ppm (1 mg/m3), skin, MAY2012 | |
| Occupational Exposure Limit-KOREA | time-weighted average 0.15 ppm (1 mg/m3), skin, 2006 | |
| Occupational Exposure Limit-NEW ZEALAND | time-weighted average 0.15 ppm (1 mg/m3), skin, JAN2002 | |
| Occupational Exposure Limit-PERU | time-weighted average 0,15 ppm (1 mg/m3), JUL2005 | |
| Occupational Exposure Limit-SWEDEN | time-weighted average 0.15 ppm (1 mg/m3);short term exposure limit 0.3 ppm (2 mg/m3), Skin, JUN2005 | |
| Occupational Exposure Limit-SWITZERLAND | MAK-week 0.15 ppm (1 mg/m3), KZG-week 0.3 ppm (2 mg/m3), skin, JAN2011 | |
| Occupational Exposure Limit-THE NETHERLANDS | MAC-TGG 1 mg/m3, 2003 | |
| Occupational Exposure Limit-UNITED KINGDOM | time-weighted average 0.15 ppm (1 mg/m3);short term exposure limit 0.5 ppm (3.5 mg/m3), skin, OCT2007 | |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction) | 8H time-weighted average 1 mg/m3 (skin) | CFRGBR 29,1926.55,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors) | 8H time-weighted average 1 mg/m3 (skin) | CFRGBR 41,50-204.50,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry) | 8H time-weighted average 1 mg/m3 (skin) | CFRGBR 29,1910.1000,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards) | 8H time-weighted average 1 mg/m3 (skin) | CFRGBR 29,1915.1000,1993 |
NIOSH Documentation and Surveillance
| Organization | Standard | Reference |
|---|---|---|
| National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO m-DINITROBENZENE-air | 10H time-weighted average 1 mg/m3 (Sk) | NIOSH* DHHS #92-100,1992 |
| National Occupational Exposure Survey 1983 | Hazard Code 27046; Number of Industries 2; Total Number of Facilities 41; Number of Occupations 4; Total Number of Employees Exposed 2489; Total Number of Female Employees Exposed 1914 | |
| National Occupational Hazard Survey 1974 | Hazard Code 27046; Number of Industries 2; Total Number of Facilities 184; Number of Occupations 3; Total Number of Employees Exposed 1754 |
Status in Federal Agencies
Page last reviewed: November 16, 2018
Content
source: National Institute for Occupational Safety and Health