Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 1—January 2021
Research

Performance of Nucleic Acid Amplification Tests for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Prospectively Pooled Specimens

Hannah Wang, Catherine A. Hogan, Jacob A. Miller, Malaya K. Sahoo, ChunHong Huang, Kenji O. Mfuh, Mamdouh Sibai, James Zehnder, Brendan Hickey, Nasa Sinnott-Armstrong, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (H. Wang, C.A. Hogan, J.A. Miller, M.K. Sahoo, C. Huang, J. Zehnder, N. Sinnott-Armstrong, B.A. Pinsky); Stanford Health Care, Stanford (K.O. Mfuh, M. Sibai, B.A. Pinsky); Stanford University, Stanford (N. Sinnott-Armstrong)

Main Article

Figure 1

Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. For a pool size of 8, paired individual and pooled Ct values for each individually positive sample (n = 58), in order of increasing individual Ct value. A) Pools comprising only 1 positive sample/pool. B) Pools comprising >2 positive samples/pool. The gray lines span the range of Ct values associated with a given pool. Rows without gray lines indicate individually positive samples belonging to pools that were negative by both real-time reverse transcription PCR methods. Panther Fusion is from Hologic (https://www.hologic.com). Ct, cycle threshold; LDT, laboratory-developed test.

Figure 1. Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. For a pool size of 8, paired individual and pooled Ct values for each individually positive sample (n = 58), in order of increasing individual Ct value. A) Pools comprising only 1 positive sample/pool. B) Pools comprising >2 positive samples/pool. The gray lines span the range of Ct values associated with a given pool. Rows without gray lines indicate individually positive samples belonging to pools that were negative by both real-time reverse transcription PCR methods. Panther Fusion is from Hologic (https://www.hologic.com). Ct, cycle threshold; LDT, laboratory-developed test.

Main Article

Page created: September 29, 2020
Page updated: December 21, 2020
Page reviewed: December 21, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external