What to know
- The content below is a compilation of materials for cholera laboratory testing.
- Resources include job aids, rapid diagnostic procedures, and diagnostic methods in English, French, and Spanish.
- Some content is aimed at countries with minimal laboratory capacity.
Laboratory methods
This manual is to be used in English- and French-speaking countries with minimal laboratory capacity and few resources. It focuses on the epidemiology of cholera, the laboratory methods to isolate and identify Vibrio cholerae serogroups O1 and O139, and how to test the susceptibility of Vibrio cholerae to antimicrobial agents in an epidemic setting.
English
- Chapter 1: The Public Health Role of Clinical Laboratories
- Chapter 2: Collection and Transport of Fecal Specimens
- Chapter 5: Etiology and Epidemiology of Cholera
- Chapter 6: Isolation and Identification of Vibrio cholerae Serogroups O1 and O139
- Chapter 10: Storage of Isolates
- Chapter 11: Quality Control of Media and Reagents
- Chapter 12: Standard Safety Practices in the Microbiology Laboratory
- Chapter 13: Packing and Shipping of Clinical Specimens and Etiologic Agents
French
- Chapitre 1: Rôle des laboratoires cliniques en santé publique
- Chapitre 2: Prélèvement et transport des échantillons de matières fécales
- Chapitre 5: Étiologie et épidémiologie du choléra
- Chapitre 6: Isolement et identification de V. cholerae O1 et O139
- Chapitre 10: Conservation des isolements
- Chapitre 11: Contrôle de qualité des milieux et réactifs
- Chapitre 12: Pratiques de sécurité standard dans le laboratoire de microbiologie
- Chapitre 13: Emballage et expédition d'échantillons cliniques ou d'agents étiologiques
English
This manual is intended for use by reference laboratories.
- Chapter 2: The Role of the Public Health Laboratory
- Chapter 3: Collection and Transport of Patient Specimens
- Chapter 4: Isolation of Vibrio cholerae from Fecal Specimens
- Chapter 6: Laboratory Identification of Vibrio cholerae
- Chapter 11: Preparation of Media and Reagents
- Chapter 12: Storage and Shipment of Isolates
- Chapter 13: Laboratory Safety
Spanish
- Capítulo 2: La función del laboratorio de salud pública
- Capítulo 3: Obtención y transporte de las muestras de pacientes
- Capítulo 4: Aislamiento de Vibrio cholerae a partir de muestras fecales
- Capítulo 6: Identificación de Vibrio cholerae en el laboratorio
- Capítulo 11: Preparación de medios de cultivo y reactivos
- Capítulo 12: Almacenamiento y envío de los aislamientos
- Capítulo 13: Aspectos de seguridad en el laboratorio