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Test Order
Test Order
Arbovirus Isolation CDC-10566
Synonym(s)
Arbovirus
CDC Pre-Approval Needed
Supplemental Information Required
Onset of symptoms date, specimen collection date, brief clinical summary, suspected agent, preliminary results, travel dates, and travel location(s).
Supplemental Form
None
Performed on Specimens from
Human
Acceptable Sample/ Specimen Type for Testing
Fresh-frozen (-20°C or lower) serum and cerebrospinal fluid (CSF). All specimens should be acute (0-7) days post onset of symptoms date for most arboviruses.
Minimum Volume Required
0.5 mL
Collection, Storage, and Preservation of Specimen Prior to Shipping
Collect blood in a serum-separator tube and separate the serum from the pelleted red blood cells following the manufacturer’s instructions for the tube used. Aseptically transfer serum to a sterile tube that has an externally threaded cap with an o-ring seal. Promptly freeze (-20°C or lower) serum specimen. For CSF, collect each specimen in a sterile leak-proof container and immediately freeze (-20°C or lower) specimen. Specimen may be stored frozen (-20°C or lower) for up to 90 days post-collection.
Transport Medium
Not Applicable
Specimen Labeling
Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. All specimens should be submitted to CDC through state health departments and not submitted directly to CDC.
Ship frozen specimens on dry ice.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
Arbovirus Diagnostic and Reference Laboratory
3156 Rampart Rd
Fort Collins, CO 80521
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
Arbovirus Diagnostic and Reference Laboratory
3156 Rampart Rd
Fort Collins, CO 80521
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Methodology
Viral isolation in cell culture
Turnaround Time
4 Weeks
Interferences & Limitations
Specimen must remain frozen (-20°C or lower); warming or freeze thawing cycles reduces sensitivity.
Additional Information
Turnaround time will vary based on length of time required for virus growth.
CDC Points of Contact
Version
1.1
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