Test Order

Yersinia (non-Y. pestis) and Other Enterobacterales Special Study
CDC-10555

Synonym(s)

CDC Pre-Approval Needed

Christine Lee
(404) 498-2295
pfx6@cdc.gov

Monica Im
(404) 718-1446
ixi9@cdc.gov

Supplemental Information Required

Complete one CDC 50.34 Specimen Submission Form per specimen and provide the following specimen information: "Specimen source (type)" and "Transport medium/Specimen preservative." Provide any preliminary results in the "Previous laboratory results" section of the CDC 50.34 Specimen Submission Form. For submissions of sequence data (e.g data_sequence specimen type) the specimen identifier should be the PNUSA number.

Supplemental Form

None

Performed on Specimens from

Human, Animal, and Food/Environmental/Medical Devices/Biologics

Acceptable Sample/ Specimen Type for Testing

Isolates, Sequence Data

Minimum Volume Required

Not Applicable

Collection, Storage, and Preservation of Specimen Prior to Shipping

Store isolates at refrigerated (2-8°C) to room temperature (15-25 °C). Isolates held for more than a month should be frozen (-20°C or lower).

Transport Medium

If isolates are shipped at room temperature or refrigerated, inoculate on nutrient agar or other similar non-selective agar (trypticase soy agar, heart infusion agar, etc.); if isolates are shipped frozen, suspend bacterial growth in trypticase soy broth (TSB) supplemented with 20% glycerol.

Specimen Labeling

Research or surveillance specimens may be labeled according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.

Shipping Instructions which Include Specimen Handling Requirements

CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Ship slants refrigerated with refrigerated or frozen cold packs or room temperature with room-temperature cold packs. Ship glycerol stocks frozen with dry ice. There are no time constraints for submitting sequence data.

Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 16
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]

All samples must be shipped in accordance with all applicable local, state and federal regulations.

Methodology

Yersinia serotyping and biotyping, Phenotypic or Genetic Subtyping

Turnaround Time

10 Weeks

Interferences & Limitations

Repeat freeze/thaw cycles can lower test sensitivity.

Additional Information

Turnaround times for special study isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

CDC Points of Contact