Test Order

Test Order

Adenovirus Molecular Detection CDC-10401

Synonym(s)
Human adenovirus, HAdV

CDC Pre-Approval Needed
David Lowe
(404) 718-6814
nqu9@cdc.gov
Lijuan Wang
(404) 639-4384
ynx2@cdc.gov

Supplemental Information Required
None

Supplemental Form

Performed on Specimens from
Human

Acceptable Sample/ Specimen Type for Testing
Nasopharyngeal swab (NP) in viral transport media (VTM), oropharyngeal swab (OP) in VTM, combined NP/OP in VTM, nasal aspirate, nasal wash, nasopharyngeal aspirate, bronchial wash, sputum, bronchoalveolar lavage (BAL), tracheal aspirate (TA), plasma, serum, blood, tissue, eye swab in VTM, and urine.

Minimum Volume Required
0.2 mL; 0.5 mL preferred

Collection, Storage, and Preservation of Specimen Prior to Shipping
Specimens should be collected as soon as possible in the course of the illness and within 72 hours of symptom onset, prior to treatment, if possible. Prior to shipment, specimens can be stored refrigerated at 2-8°C for up to 7 days after collection; specimens stored for longer than 7 days should be stored frozen at -20°C or lower for up to 2 months. Liquid specimens should be aliquoted into properly labeled, leak-proof, unbreakable screw cap vials.

Transport Medium
Viral transport medium (VTM) should be used with specimen types: nasopharyngeal swabs (NP), oropharyngeal swabs (OP), nasopharyngeal/oropharyngeal swabs (NP/OP), and eye swabs.

Specimen Labeling
This test order can accommodate diagnostic and non-diagnostic specimens. Testing subject to CLIA regulations requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition. Research or surveillance specimens may be labeled according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.

Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Specimens should be shipped frozen, overnight on dry ice.

Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 84
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]

All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.


Methodology
Polymerase Chain Reaction (PCR)

Turnaround Time
2 Weeks

Interferences & Limitations
Use only synthetic fiber swabs with thin plastic or wire shafts that have been designed for sampling the nasopharyngeal mucosa. Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inactivate some viruses and may inhibit molecular tests.



Whole blood or plasma specimens should be collected in a purple top EDTA tube or a yellow top Acid Citrate Dextrose (ACD) tube. Other acceptable collection tubes include pearl top Plasma Preparation Tube (PPT) and tiger top serum separator tube (SST). Heparin tubes are not recommended for molecular testing as heparin can interfere with PCR reactions.

Additional Information
None

CDC Points of Contact
Lijuan Wang
(404) 639-4384
ynx2@cdc.gov
Stacey Gonder
(404) 639-8739
urv6@cdc.gov
David Lowe
(404) 718-6814
nqu9@cdc.gov

Version
3.9