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Polio Isolation and Genotyping
CDC-10376
Synonym(s)
PV, polio virus, Polio sequencing, AFP, acute flaccid paralysis
CDC Pre-Approval Needed
None
Supplemental Information Required
None
Supplemental Form
None
Performed on Specimens from
Human, Animal, and Food/Environmental/Medical Devices/Biologics
Acceptable Sample/ Specimen Type for Testing
Stool, cell culture isolate, Fast Technology for Analysis of nucleic acids (FTA) cards, wastewater
Minimum Volume Required
Tissue culture isolate: 0.5 mL
Stool: 1 gram; 10 - 20 grams preferred
Wastewater: 500mL
Stool: 1 gram; 10 - 20 grams preferred
Wastewater: 500mL
Collection, Storage, and Preservation of Specimen Prior to Shipping
Keep specimens refrigerated (2-8 °C) or frozen (-20 °C or lower).
For stool, do not add transport medium.
Wastewater should be stored refrigerated (2-8 °C) upon collection and stored frozen (-20 °C or lower) prior to shipping.
For stool, do not add transport medium.
Wastewater should be stored refrigerated (2-8 °C) upon collection and stored frozen (-20 °C or lower) prior to shipping.
Transport Medium
Not Applicable
Specimen Labeling
Research or surveillance specimens may be labeled with unique identifiers according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday.
Frozen specimens should be shipped on dry ice. FTA cards should be shipped at ambient temperatures and should include humidity indicator cards and desiccant pouches. When shipping ambient specimens, no temperature-maintaining materials (e.g., cold packs) are required.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 76
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Frozen specimens should be shipped on dry ice. FTA cards should be shipped at ambient temperatures and should include humidity indicator cards and desiccant pouches. When shipping ambient specimens, no temperature-maintaining materials (e.g., cold packs) are required.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 76
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Methodology
Cell culture, Molecular techniques
Turnaround Time
3 Weeks
Interferences & Limitations
None
Additional Information
If case investigation form is readily available, please submit with specimen
CDC Points of Contact
Version
1.4
Content Credential