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Test Order
Test Order
Picornavirus Detection and Identification (not Hepatitis A, not Rhinovirus) CDC-10374
Synonym(s)
Theier's murine encephalomyelitis virus (TMEV), Saffold virus (SAFV), Cosavirus (COSV) (Dekavirus), Salivirus (SALV) (Klassevirus), Kobuvirus, Aichi virus, Encephalomyocarditis virus (EMCV), Vilyuisk virus
Test Order Status
This Test Order is currently accepting specimens.
CDC Pre-Approval Needed
Supplemental Information Required
None
Supplemental Form
None
Performed on Specimens from
Human, Animal, and Food/Environmental/Medical Devices/Biologics
Acceptable Sample/ Specimen Type for Testing
Stool, Cerebrospinal fluid (CSF), Serum, Respiratory swab specimens in virus transport media (VTM), including nasopharyngeal swab (NP), oropharyngeal swab (OP), nasopharyngeal/oropharyngeal swab (NP/OP), nasal swab (NS), Respiratory wash specimens, including bronchoalveolar lavage (BAL), bronchial wash (BW), nasal wash (NW), tracheal aspirate (TA), nasal aspirate (NA), Rectal swab in virus transport media (VTM), Conjunctival swab in VTM, Lesion swab in VTM.
Minimum Volume Required
Stool: 1 gram, 10 - 20 grams preferred
Cerebrospinal fluid (CSF): 0.15 mL, 0.5-2 mL preferred
Serum: 0.15 mL, 0.5 - 2 mL preferred
Respiratory wash specimens and swab specimens in virus transport media: 0.5 mL, 1 mL preferred
Rectal, conjunctival, and lesion swab in virus transport media: 0.5 mL, 1 mL preferred
Cerebrospinal fluid (CSF): 0.15 mL, 0.5-2 mL preferred
Serum: 0.15 mL, 0.5 - 2 mL preferred
Respiratory wash specimens and swab specimens in virus transport media: 0.5 mL, 1 mL preferred
Rectal, conjunctival, and lesion swab in virus transport media: 0.5 mL, 1 mL preferred
Collection, Storage, and Preservation of Specimen Prior to Shipping
Collecting specimens upon the first week of illness is ideal; if collected the second week, it should include a stool sample.
For all swab specimens, use only sterile Dacron or rayon swabs with plastic shafts or, if available, flocked swabs. Place the swab immediately into a sterile vial containing 2 mL of viral transport media.
For stool, CSF, and respiratory wash specimens, collect each specimen in a clean, dry, leak-proof container. Stool should be collected within 14 days of symptom onset. Do not add transport medium.
For serum specimens, collect whole blood into a serum separator tube (marble or tiger top SST). Allow to clot at room temperature (15°C to 25°C) for a minimum of 30 minutes and centrifuge.
After collection, freeze (-20°C or lower) all specimens and ship to CDC within 2 months. Please note: If necessary, CSF, conjunctival swabs and lesion swabs may be kept at 2-8°C for no more than 72 hours after collection and prior to freezing.
If necessary, stools, serum, respiratory swabs and washes, and rectal swabs may be kept at 2-8°C for no more than 14 days after collection and prior to freezing.
For all swab specimens, use only sterile Dacron or rayon swabs with plastic shafts or, if available, flocked swabs. Place the swab immediately into a sterile vial containing 2 mL of viral transport media.
For stool, CSF, and respiratory wash specimens, collect each specimen in a clean, dry, leak-proof container. Stool should be collected within 14 days of symptom onset. Do not add transport medium.
For serum specimens, collect whole blood into a serum separator tube (marble or tiger top SST). Allow to clot at room temperature (15°C to 25°C) for a minimum of 30 minutes and centrifuge.
After collection, freeze (-20°C or lower) all specimens and ship to CDC within 2 months. Please note: If necessary, CSF, conjunctival swabs and lesion swabs may be kept at 2-8°C for no more than 72 hours after collection and prior to freezing.
If necessary, stools, serum, respiratory swabs and washes, and rectal swabs may be kept at 2-8°C for no more than 14 days after collection and prior to freezing.
Transport Medium
Viral transport medium (VTM) should be used with these specimen types: nasopharyngeal swabs (NP), oropharyngeal swabs (OP), nasopharyngeal/oropharyngeal swabs (NP/OP), nasal swabs (NS), rectal swabs, conjunctival swab, and lesion swabs.
Specimen Labeling
Research or surveillance specimens may be labeled with unique identifiers according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Frozen specimens should be shipped on dry ice. All specimens should be shipped frozen on dry ice under UN3373, Category B.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 76
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 76
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Methodology
Molecular techniques
Turnaround Time
Interferences & Limitations
Frozen specimens must remain frozen; warming or freeze-thaw cycle reduces sensitivity. For serum, heparin may cause interference with the molecular tests and should be avoided. For swab specimens, do not use calcium alginate swabs or swabs with wooden sticks, as they may inactivate some viruses and inhibit some molecular assays.
Additional Information
Not Applicable
CDC Points of Contact
Version
1.6
Content Credential