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Test Order
Test Order
Arbovirus Serology CDC-10282
Synonym(s)
Arbovirus, Arbo serology, Arbovirus immunoglobulin M (IgM), Eastern equine encephalitis virus (EEEV), Jamestown Canyon virus (JCV), La Crosse virus (LACV), Powassan virus (POWV), St. Louis encephalitis virus (SLEV), West Nile virus (WNV), Yellow fever virus (YFV), Zika virus (ZIKV)
CDC Pre-Approval Needed
None
Supplemental Information Required
Onset date, specimen collected date, brief clinical summary, suspected agent, travel dates, and travel location(s)
Supplemental Form
None
Performed on Specimens from
Human
Acceptable Sample/ Specimen Type for Testing
Serum and cerebrospinal fluid (CSF)
Minimum Volume Required
0.5 mL
Collection, Storage, and Preservation of Specimen Prior to Shipping
Collect blood in a serum-separator tube and separate the serum from the pelleted red blood cells following the manufacturer’s instructions for the tube used. Aseptically transfer serum to a sterile tube that has an externally threaded cap with an o-ring seal. Promptly refrigerate (2-8°C) or freeze (-20°C or lower) serum specimen.
For CSF, collect each specimen in a clean, dry, leak-proof container and immediately refrigerate (2-8°C) or freeze (-20°C or lower) specimen.
Specimen may be stored at refrigerated temperature (2-8°C) for up to 120 days and frozen (-20°C or lower) for up to 1 year post-collection.
Specimen must not exceed 3 freeze/thaw cycles.
For CSF, collect each specimen in a clean, dry, leak-proof container and immediately refrigerate (2-8°C) or freeze (-20°C or lower) specimen.
Specimen may be stored at refrigerated temperature (2-8°C) for up to 120 days and frozen (-20°C or lower) for up to 1 year post-collection.
Specimen must not exceed 3 freeze/thaw cycles.
Transport Medium
Not Applicable
Specimen Labeling
Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. All specimens should be submitted to CDC through state health departments and NOT submitted directly to CDC.
Ship frozen specimens on dry ice. Ship refrigerated specimens with refrigerated or frozen cold packs.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
Arbovirus Diagnostic and Reference Laboratory
3156 Rampart Rd
Fort Collins, CO 80521
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Ship frozen specimens on dry ice. Ship refrigerated specimens with refrigerated or frozen cold packs.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
Arbovirus Diagnostic and Reference Laboratory
3156 Rampart Rd
Fort Collins, CO 80521
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Methodology
Enzyme-linked immunosorbent assay (ELISA) immunoglobulin (Ig) M, Microsphere immunoassay (MIA) IgM
Turnaround Time
4 Weeks
Interferences & Limitations
Hemolysis can cause non-specific binding in serological tests and can have an effect on laboratory results.
Additional Information
IgM serology testing currently offline for many arboviruses (unless listed above in test order synonym). Diagnostic testing is available commercially and/or through Wadsworth Center Virology NYS Department of Health. Please contact CDC for assistance if needed.
Heartland virus and Japanese encephalitis virus IgM testing may be available with prior approval from Dr. Amy Lambert.
For additional information regarding the fields above, please see this link: https://www.cdc.gov/vector-borne-diseases/php/laboratories/submitting-arboviral-specimens.html
Turnaround Time is impacted by whether the specimen tests positive for immunoglobulin (Ig) M antibodies, IgM positive samples may have plaque reduction neutralization tests performed (see CDC-10283 Arbovirus Neutralization Antibodies).
Heartland virus and Japanese encephalitis virus IgM testing may be available with prior approval from Dr. Amy Lambert.
For additional information regarding the fields above, please see this link: https://www.cdc.gov/vector-borne-diseases/php/laboratories/submitting-arboviral-specimens.html
Turnaround Time is impacted by whether the specimen tests positive for immunoglobulin (Ig) M antibodies, IgM positive samples may have plaque reduction neutralization tests performed (see CDC-10283 Arbovirus Neutralization Antibodies).
CDC Points of Contact
Version
1.5
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