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Test Order
Test Order
Measles Serology CDC-10244
Synonym(s)
Rubeola
CDC Pre-Approval Needed
None
Supplemental Information Required
Provide the following information on CDC 50.34 Specimen Submission Form: date of onset, date of specimen collection, date(s) of MMR vaccination (if known) and travel history. Provide any preliminary results available.
Supplemental Form
None
Performed on Specimens from
Human
Acceptable Sample/ Specimen Type for Testing
Serum.
The following conditions may result in the specimen being rejected for testing:
• Specimen is hemolyzed, lipemic, or bacterially contaminated.
• Specimen is not frozen upon receipt at CDC.
The following conditions may result in the specimen being rejected for testing:
• Specimen is hemolyzed, lipemic, or bacterially contaminated.
• Specimen is not frozen upon receipt at CDC.
Minimum Volume Required
0.5 mL
Collection, Storage, and Preservation of Specimen Prior to Shipping
Blood for serologic testing should be collected by aseptic venipuncture. Do not add anticoagulants or preservatives. Collect blood in a red-top or serum-separator tube (SST). Tubes containing whole blood should not be stored frozen (-20°C or lower). Centrifuge blood collection tubes (10 minutes at 1000 – 1300 g) to separate serum from clot. Gel separation tubes should be centrifuged no more than 2 hours after collection. Aseptically transfer serum to a sterile tube that has an externally threaded cap with an o–ring seal.
Refrigerate serum (2-8°C) within 8 hours of collection. Serum samples should not be stored at room temperature (15-25°C) for longer than 8 hours after collection. If the samples will be stored for more than 48 hours, freeze specimens (-20°C or lower). All samples submitted for serology testing should be frozen (-20°C or lower) and shipped on dry ice. Serum specimens can be stored frozen (-20°C or lower) for up to 8 weeks prior to shipping.
Avoid multiple freeze/thaw cycles which may cause loss of antibody activity and give erroneous results.
Refrigerate serum (2-8°C) within 8 hours of collection. Serum samples should not be stored at room temperature (15-25°C) for longer than 8 hours after collection. If the samples will be stored for more than 48 hours, freeze specimens (-20°C or lower). All samples submitted for serology testing should be frozen (-20°C or lower) and shipped on dry ice. Serum specimens can be stored frozen (-20°C or lower) for up to 8 weeks prior to shipping.
Avoid multiple freeze/thaw cycles which may cause loss of antibody activity and give erroneous results.
Transport Medium
Not applicable
Specimen Labeling
Test subject to CLIA regulations and requires two patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as a medical record number) on the specimen container and on the test requisition.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Freeze serum specimens and ship to CDC frozen on dry ice overnight. Specimens should be shipped in proper secondary containment to prevent exposures due to leaks in primary tubes.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 81
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 81
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Methodology
Capture IgM Enzyme Linked Immunosorbant Assay (ELISA) and Indirect IgG ELISA
Turnaround Time
7 Days
Interferences & Limitations
IgM positive may not occur until 4 days post-rash onset
Additional Information
IgM and IgG assays are qualitative assays. Include
vaccination history, age, date of onset and sample collection.
CDC Points of Contact
Version
2.4
Content Credential