Test Order

anaerobe ID, anaerobe bacteria identification, anaerobe, anaerobic Actinomyces identification

Human

Not Applicable

Inoculate pure culture isolate in chopped meat broth and incubate anaerobically for 36-48 hours. Store anaerobically sealed vial at room temperature (15-25°C) or refrigerated temperature (2-8°C) and ship within 3 days of inoculation to transport media.

For pure culture isolate on commercial semi-solid media, store according to temperature recommendations outlined in manufacturer’s instructions and ship room temperature vials (15-25°C) or refrigerated temperature vials (2-8°C) within 3 days of inoculation to transport media.

Alternatively, freeze culture following 36-48 hour anaerobic incubation and store frozen (-20°C or lower) until shipped. Anaerobe isolates submitted frozen in tryptic soy broth (TSB) plus glycerol (or preferably sterile skim milk media plus glycerol to enhance viability) must be inoculated very heavily (half to all the colonial mass from a 48-hour anaerobe culture plate). Ship isolates as soon as possible to ensure viability.

Transport pure culture isolates in chopped meat broth at room temperature (15-25°C) or refrigerated (2-8°C).

Transport pure culture isolates in commercial anaerobic semi-solid agar tube transport media (e.g. Anaerobe Systems Transport Media) in accordance with manufacturer's instructions.

Transport pure culture isolates frozen (-20°C or lower) in sterile skim milk media plus glycerol (preferred) or tryptic soy broth (TSB) plus glycerol.

Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.

CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. For pure culture isolates, ship broths overnight at room temperature or refrigerated. For semi-solid media, ship specimens at room temperature or refrigerated in accordance with manufacturer’s instructions. Room-temperature samples should be shipped with room-temperature cold packs. Refrigerated samples should be shipped with refrigerated or frozen cold packs. Frozen samples should be shipped on dry ice. 

Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 13
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]

All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.

16S ribosomal ribonucleic acid (rRNA) gene sequencing and additional phenotypic testing

4 Weeks

Specimens from respiratory, vaginal, and fecal sources are not acceptable. Pure culture isolates must be viable for testing.

If a healthcare facility will be submitting samples directly to CDC they must receive prior approval from the State Health Department. If submitting pure culture isolate(s) for testing, the original culture/isolate/inoculum should be maintained by the submitter until results are reported, indicating that CDC testing is completed.

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