Test Order

Test Order

Staphylococcus and Micrococcus - Identification (ID)
CDC-10226

Synonym(s)
Staph, Micrococcus, Kocuria Identification

CDC Pre-Approval Needed
None

Supplemental Information Required
The CDC 50.34 Specimen Submission Form or Global File Accessioning Template (GFAT) must include the State Public Health Department contact information, as well as the date the submitted culture was inoculated onto transport media.

Supplemental Form

Performed on Specimens from
Human

Acceptable Sample/ Specimen Type for Testing
Pure culture isolates of suspected Staphylococcus spp., Micrococcus spp., Kocuria spp. and other aerobic, catalase-positive, Gram-positive cocci species

Minimum Volume Required
Not Applicable

Collection, Storage, and Preservation of Specimen Prior to Shipping
Store pure culture isolates at room temperature (15-25 °C) for up to 7 days or at refrigerated temperature (2-8 °C) up to 14 days.

Isolates being stored more than 14 days should be frozen (-20 °C or lower). Ship isolates as soon as possible to ensure viability.

Transport Medium
Transport pure culture isolates at room temperature (15-25 °C) or refrigerated (2-8 °C) on trypticase soy agar (TSA); heart or brain-heart infusion agar (HIA or BHIA); blood agar or chocolate agar.

Transport frozen (-20 °C or lower) submissions in trypticase soy broth (TSB) plus 15% glycerol.

Specimen Labeling
Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.

Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Ship pure culture isolates overnight at room temperature, refrigerated, or frozen. Room temperature samples should be shipped with room temperature cold packs. Refrigerated samples should be shipped with refrigerated or frozen cold packs. Frozen samples should be shipped frozen with dry ice.

Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 13
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]

All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.


Methodology
16S ribosomal ribonucleic acid (rRNA) gene sequencing, matrix assisted laser desorption ionization-time of flight (MALDI-TOF), additional phenotypic testing

Turnaround Time
4 Weeks

Interferences & Limitations
Pure culture isolates must be viable for testing.

Additional Information
If a Healthcare facility will be submitting samples directly to CDC they must receive prior approval from the State Health Department. If submitting pure culture isolate(s) for testing, the original culture/isolate/inoculum should be maintained by the submitter until results are reported, indicating that CDC testing is completed.

CDC Points of Contact
Stephen LaVoie
(404) 718-4747
qea5@cdc.gov
Davina Campbell
(404) 639-4185
xew9@cdc.gov

Version
2.4