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Test Order
Test Order
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This test order is unavailable until further notice.
Neisseria meningitidis Identification and Serogrouping CDC-10219
Synonym(s)
N. meningitidis ID and Serogrouping, Nm ID
CDC Pre-Approval Needed
None
Supplemental Information Required
Two primary patient identifiers are required for this test order.
Provide any preliminary results available (including manufacturer of antiserum or PCR methods used if applicable) in the Previous Laboratory Results section on the CDC 50.34 Specimen Submission Form.
If prioritized testing is needed for a public health response, contact CDC POC for approval of expedited testing.
For surveillance testing, please submit under the Neisseria meningitidis Surveillance test order (CDC-10220).
Supplemental Form
None
Performed on Specimens from
Human
Acceptable Sample/ Specimen Type for Testing
This Test Order is currently not accepting any specimens until further notice.
Minimum Volume Required
0.25 mL for frozen bacterial stocks; 0.5 mL or more is preferred for primary specimens
Collection, Storage, and Preservation of Specimen Prior to Shipping
Bacterial isolate stocks should be stored in a cryovial and kept frozen (-20°C or lower) prior to shipping.
When submitting viable bacterial isolates, incubate the inoculated chocolate agar slants overnight at 35-39°C with 5% carbon dioxide to ensure viability of the isolates.
Cerebrospinal fluid (CSF) and serum should be refrigerated (2-8°C) after collection and frozen (-20°C or lower) within 96 hours for up to 60 days.
When submitting viable bacterial isolates, incubate the inoculated chocolate agar slants overnight at 35-39°C with 5% carbon dioxide to ensure viability of the isolates.
Cerebrospinal fluid (CSF) and serum should be refrigerated (2-8°C) after collection and frozen (-20°C or lower) within 96 hours for up to 60 days.
Transport Medium
When submitting frozen bacterial stocks, use defibrinated sheep blood or trypticase soy broth (TSB) plus 15% glycerol. When submitting viable bacterial cultures at room temperature, use chocolate agar slants.
Specimen Labeling
Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. If shipping viable bacterial isolates, ship at room temperature with room-temperature cold packs. When shipping 10 or more bacterial isolates, submit frozen stocks only. Frozen bacterial isolate stocks and primary specimens (CSF and serum) should be shipped on dry ice and received frozen.
Enclose CDC 50.34 Specimen Submission Form in shipment. Email the tracking number in advance, particularly if prioritized testing is requested.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 10
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Enclose CDC 50.34 Specimen Submission Form in shipment. Email the tracking number in advance, particularly if prioritized testing is requested.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 10
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Methodology
Bacterial isolates and primary specimens will be characterized using real-time polymerase chain reaction (PCR).
Turnaround Time
4 Weeks
Interferences & Limitations
Bacterial DNA concentration, low specimen volume, collection time, and transport and
handling conditions may impact the results. Primary specimens that were collected after antibiotic treatment, were transported under suboptimal conditions, or have a particularly low volume and/or bacterial DNA load may result in a false negative result.
Additional Information
Test results provide or confirm serogroup of N. meningitidis.
CDC Points of Contact
Version
2.7
Content Credential