Test Order

Test Order

Mycobacterium TB Complex - Identification and Pyrazinamide Susceptibility Testing
CDC-10190

Synonym(s)
MTB DST, TB, Tuberculosis, MTBC

CDC Pre-Approval Needed
None

Supplemental Information Required
Provide the following Specimen Information on the CDC 50.34 Specimen Submission Form: material submitted (bacterial isolate), the specimen source (type), specimen collection date, date sent to CDC, and transport medium/specimen preservative.

Supplemental Form

Performed on Specimens from
Human

Acceptable Sample/ Specimen Type for Testing
This test order is currently not accepting any specimens until further notice.

Minimum Volume Required
0.5 mL, 1 mL is preferred

Collection, Storage, and Preservation of Specimen Prior to Shipping
Solid media: store refrigerated (2-8°C) or room temperature (15-25°C) up to 180 days post collection

Liquid media: store refrigerated (2-8°C), room temperature (15-25°C), frozen (-20°C or lower) up to 120 days post collection. Store in deep freeze (-70°C or lower) up to 5 years post collection

Transport Medium
Solid media (i.e., Middlebrook 7H10 or 7H11 plates, Lowenstein-Jensen (LJ) slants) Liquid media (i.e., 7H9, Mycobacterial Growth Indicator Tube (MGIT), BACT/ALERT, VersaTREK) or MTBC colony growth suspended in saline or water

Specimen Labeling
Test subject to CLIA regulations and requires two primary patient identifiers (e.g., patient first and last name, date of birth, unique patient identifier from time of collection, such as medical record number) on the specimen container and on the test requisition.

Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Ship at room temperature with room-temperature cold packs; must be received within 5 days of shipment. Submit liquid medium in a screwcap cryovial that has been sealed with parafilm; do not send in a 15- or 50-mL conical tube. Broth should not be shipped frozen. MTBC isolates must be shipped as Infectious substances (Div. 6.2, Class Category A).

Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 29
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]

All samples must be shipped in accordance with all applicable local, state and federal regulations.


Methodology
DNA sequencing for Pyrazinamide (PZA) susceptibility, Genetic based testing (ID)

Turnaround Time
4 Weeks

Interferences & Limitations
Contaminated samples (i.e., not a pure culture of MTB) for PZA by MGIT 960 are reported as contaminated and additional submission of pure culture may be clinically needed.

Additional Information
None

CDC Points of Contact
TB Lab
(404) 639-2455
TBLab@cdc.gov
David Sikes
(404) 639-5489
zrg5@cdc.gov

Version
2.9