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Test Order
Test Order
Healthcare-Associated Infections (HAI) - Outbreak Investigation CDC-10162
Synonym(s)
Healthcare Outbreak, Nosocomial Outbreak, Healthcare-Associated Infection (HAI) Outbreak
CDC Pre-Approval Needed
Supplemental Information Required
Contact the CDC POC for instructions on completing a Supplemental Line List.
The CDC 50.34 Specimen Submission Form or Global File Accessioning Template (GFAT) or Supplemental Line List must include the date the submitted culture was inoculated onto transport media and/or the date environmental samples were collected.
The CDC 50.34 Specimen Submission Form or Global File Accessioning Template (GFAT) or Supplemental Line List must include the date the submitted culture was inoculated onto transport media and/or the date environmental samples were collected.
Supplemental Form
Contact the CDC POC regarding contents of the required Supplemental Line List.
Performed on Specimens from
Human and Food/Environmental/Medical Devices/Biologics
Acceptable Sample/ Specimen Type for Testing
Acceptable samples include pure culture isolates and primary environmental specimen types (e.g. swabs, wipes, water and other fluids, medical devices, products), however, additional sample types may be accepted for testing upon consultation.
Minimum Volume Required
Not applicable for pure culture isolates. Minimum volumes may be required for certain types of environmental samples. Please confirm volume requirements with the CDC POC before collecting samples.
Collection, Storage, and Preservation of Specimen Prior to Shipping
Store all aerobic bacterial isolates at room temperature (15-25°C). If isolates cannot be shipped within 24 hours, refrigerate only non-fastidious organisms (2-8°C) for shipping.
Primary environmental specimens should be refrigerated (2-8°C) within 1 hour. Samples of healthcare or outbreak related products (e.g. compounded medications, lotions, medical devices) should be stored in accordance with manufacturer's instructions if relevant. Special storage and preservation requirements for anaerobic isolates and other unlisted environmental or product samples are available upon request.
Primary environmental specimens should be refrigerated (2-8°C) within 1 hour. Samples of healthcare or outbreak related products (e.g. compounded medications, lotions, medical devices) should be stored in accordance with manufacturer's instructions if relevant. Special storage and preservation requirements for anaerobic isolates and other unlisted environmental or product samples are available upon request.
Transport Medium
Transport pure culture isolates at room temperature (15-25 °C) or refrigerated (2-8 °C) on suitable agar slant (not an agar plate or broth culture media). Transport frozen (-20 °C or lower) submissions in trypticase soy broth (TSB) plus 15% glycerol.
Transport refrigerated (2-8 °C) environmental specimens in suitable buffers or media if necessary (e.g. swabs). Transport conditions for anaerobic isolates available upon request.
Transport refrigerated (2-8 °C) environmental specimens in suitable buffers or media if necessary (e.g. swabs). Transport conditions for anaerobic isolates available upon request.
Specimen Labeling
Research or surveillance specimens may be labeled according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Ship submissions overnight. Room-temperature samples should be shipped with room-temperature cold packs. Refrigerated samples should be shipped with refrigerated or frozen cold packs. Frozen samples should be shipped frozen with dry ice.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 154
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 154
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations. Upon shipment, submitter should send an email to the CDC POC providing shipping company, shipped date and package tracking number.
Methodology
Molecular Identification and Typing of non-clinical specimens (e.g., MALDI-ToF, 16S, Pulsed-Field Gel Electrophoresis (PFGE), Whole Genome Sequencing (WGS)), Primary Processing of non-clinical specimens (e.g., culture and isolation, species identification, membrane filtration, sterility testing)
Turnaround Time
Interferences & Limitations
Primary environmental samples should not be held at room temperature for >1 hour. Doing so may decrease recovery of microorganisms or otherwise adversely affect the results obtained from testing recovered organisms. Neutralization of chlorine residual in potable water is necessary during collection. Samples that arrive containing personally identifiable information under this test code will be rejected for testing. Pure culture isolates must be viable for testing.
Additional Information
Contact the CDC POC for approval prior to submitting any specimen. If a Healthcare facility will be submitting samples directly to CDC they must receive prior approval from the State Health Department. Prior epidemiologic consultation with CDC/DHQP Prevention and Response Branch [haioutbreak@cdc.gov] also required.
Turnaround Times vary based on target organism and sample type.
The test(s) used have not been cleared and approved by the FDA or the performance characteristics have not been fully established by CDC. The results should NOT be used for diagnosis, treatment, or assessment of patient health or management.
If submitting pure culture isolate(s) for testing, the original culture/isolate/inoculum should be maintained by the submitter until results are reported, indicating that CDC testing is completed.
Turnaround Times vary based on target organism and sample type.
The test(s) used have not been cleared and approved by the FDA or the performance characteristics have not been fully established by CDC. The results should NOT be used for diagnosis, treatment, or assessment of patient health or management.
If submitting pure culture isolate(s) for testing, the original culture/isolate/inoculum should be maintained by the submitter until results are reported, indicating that CDC testing is completed.
CDC Points of Contact
Version
3.4
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