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Test Order
Test Order
Escherichia coli and Shigella Subtyping
CDC-10116
Synonym(s)
Escherichia, STEC, Shigella, E. coli, subtyping
CDC Pre-Approval Needed
None
Supplemental Information Required
Complete one CDC 50.34 Specimen Submission Form per specimen and provide the following specimen information: "Specimen source (type)" and "Transport medium/Specimen preservative." Provide any preliminary results in the "Previous laboratory results" section of the CDC 50.34 Specimen Submission Form. For submissions of sequence data (e.g. data_sequence specimen type) the specimen identifier should be the PNUSA number. Specify type of subtyping requested in "Previous Laboratory Results" on back of CDC 50.34 Specimen Submission Form. Epidemiologic metadata and PulseNet cluster code are requested if available.
Supplemental Form
None
Performed on Specimens from
Human, Animal, and Food/Environmental/Medical Devices/Biologics
Acceptable Sample/ Specimen Type for Testing
Pure culture isolates of Escherichia and Shigella; Sequence Data
Minimum Volume Required
No minimum volume required.
Collection, Storage, and Preservation of Specimen Prior to Shipping
Store isolates at refrigerated (2-8°C) to room temperature (15-25 °C). Isolates held for more than a month should be frozen (-20°C or lower).
Transport Medium
If isolates are shipped at room temperature or refrigerated, inoculate on nutrient agar or other similar non-selective agar (trypticase soy agar, heart infusion agar, etc.); if isolates are shipped frozen, suspend bacterial growth in trypticase soy broth (TSB) supplemented with 20% glycerol.
Specimen Labeling
Research or surveillance specimens may be labeled according to protocol. Labels should not include personally identifiable information. The results reported should NOT be used for diagnosis, treatment, assessment of health or management of the individual patient.
Shipping Instructions which Include Specimen Handling Requirements
CDC does not accept routine shipments on weekends or holidays. Please make sure packages arrive Monday – Friday. Ship slants refrigerated with refrigerated or frozen cold packs or room temperature with room-temperature cold packs. Ship glycerol stocks frozen with dry ice. Shiga toxin-positive bacteria should be shipped as Category A Infectious Substances. There are no time constraints for submitting sequence data.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 07
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Ship To:
[Insert CDC Point of Contact]
Centers for Disease Control and Prevention
RDSB/STATT Unit 07
1600 Clifton Road, NE
Atlanta, GA 30329
[Insert CDC Point of Contact’s Telephone Number]
All samples must be shipped in accordance with all applicable local, state and federal regulations.
Methodology
Phenotypic or Genetic Identification and Subtyping, including Serotyping and Virulence Profiling, Antimicrobial Susceptibility Testing (AST)
Turnaround Time
20 Weeks
Interferences & Limitations
Virulence and serotype modification genes encoded by mobile genetic elements (bacteriophages, plasmids and pathogenicity islands) may be spontaneously lost during transit and storage. Conditions that induce a stress response in the bacterium can affect the stability of virulence factors and may affect the expression of O and H antigens.
Additional Information
Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.
CDC Points of Contact
Version
1.7
Content Credential